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    Florida Medical Entomology Laboratory

    Florida Medical Entomology Laboratory

    Species Identification Tables: Larvae

    Click on column species names for images

    • Aedes
    • Anopheles
    • Culex
    • Mansonia & Coquillettidia
    • Psorophora
    • Uranotaenia
    • Wyeomyia

    Aedes

    characters Ae aegypti Ae albopictus Ae vexans Ae bahamensis Ae fulvus pallens Ae infirmatus Ae sollicitans Ae taeniorhynchus Ae tormentor Ae triseriatus
    ANTENNAE single hair near middle single hair near saddle hair tuft near middle   hair tuft near middle hair tuft near middle hair tuft near middle hair tuft with only 2-3 branches, near middle hair tuft near middle single hair near middle
    HEAD upper & lower hairs single upper & lower hairs single upper & lower hairs multiple   upper hair single, lower hair triple upper & lower hairs single upper & lower hairs single upper & lower hairs single upper & lower hairs single upper hair single, lower hair 2 or more branches
    THORAX heavy hooks on sides weak or no hooks on side                
    ABDOMEN     side hairs on segments III-V double or triple many stellate hair tufts            
    COMB SCALES pitchfork-shaped; 7-12 in 1 row thorn-like; in 1 row thorn-like; 9-12 in roughly 2 rows rounded at tip; 10-18 in 1 row rounded at tip; 25+ in a patch thorn-like; 15-22 in a patch thorn-like; 11-22 in a patch rounded at tip; 9-20 in a patch thorn-like; 9-12 in 1 irregular curved row thick, blunt; 8-15 in 1 uneven row
    SIPHON index 2.0; pecten evenly spaced pecten evenly spaced index 3.0-3.5; pecten teeth not evenly spaced, last tooth widely spaced index 2.0-2.4; pecten evenly spaced index 2.0; last pecten tooth detached; hair tuft inserted within pecten index 2.5; pecten evenly spaced; preapical spine not longer than 1/2 length of apical pecten tooth index 2.0-2.5; pecten evenly spaced index <2.0; pecten evenly spaced index 2.0; pecten evenly spaced; hair tuft inserted within pecten index 2.5-3.0; pecten evenly spaced
    SADDLE not completely ringed not completely ringed not completely ringed not completely ringed; spines on edge completely ringed completely ringed completely ringed completely ringed completely ringed not completely ringed
    GILLS all the same length, rounded; longer than anal segment all the same length, rounded; longer than anal segment all the same length, pointed; longer than anal segment different lengths, rounded; about as long as anal segment all the same length, pointed; longer than anal segment all the same length, pointed; longer than anal segment variable in length, rounded; usually shorter than anal segment all the same length; rounded; shorter than anal segment variable in length, pointed; longer than anal segment different lengths; rounded, bud-like; about same length as anal segment

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    Anopheles

    characters An crucians An quadrimaculatus An walkeri
    ANTENNAE hair tuft inserts at basal 1/3 hair tuft inserts near middle hair tuft inserts at basal 1/3
    HEAD hair #3 multi-branched; #2 simple hair #3 multi-branched; #2 simple hair #3 multi-branched; #2 has sparse feathering toward the tip
    ABDOMEN (palmate hair) reduced on segments I & II; well-developed on segments III-VII reduced on segments I & II; well developed on III-VII partially developed on segments I & II; well-developed on III-VII
    ABDOMEN (hair #0 and hair #2) #0 & #2 multi-branched & almost equal in size on segments IV & V #0 very small, single and #2 single on segments IV & V #0 smaller than #2, branched and #2 single on segments IV & V

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    Culex

    characters Cx biscaynensis Cx declarator Cx erraticus Cx nigripalpus Cx pilosus Cx quinquefasciatus Cx restuans Cx salinarius Cx territans
    HEAD upper & lower hairs have 5 or more branches upper & lower hairs triple upper hair short with multiple branches; lower hair single upper & lower hairs usually have 3 branches, sometimes 4 upper & lower hairs single; ventral side of head has ovoid gills near antennae upper & lower hairs have 5 or more branches upper & lower hairs have 5 or more branches; hair tuft near middle of antennae upper hair has 3-6 branches; lower hair has 3-4 branches upper & lower hairs single
    ABDOMEN many stellate hair tufts   4th segment clear, most prominent in early instars 4th segment clear, most prominent in early instars         4th segment clear, most prominent in early instars
    COMB SCALES blunt; 37-55 in a patch blunt; many in a patch thorn-shaped; many in an irregular single or double row blunt; many in a patch thorn-shaped; 8-12 in 1 irregular row blunt; many in a patch blunt; many in a patch blunt; many in a patch blunt; many in a patch
    ANAL SEGMENT spines on edge of saddle; lateral hair single lateral hair single lateral hair short, single or double lateral hair single lateral hair double or triple lateral hair single lateral hair single or double; dorsal hair double lateral hair double lateral hair double
    SIPHON index 9.2-12.2; long, needle-like; subdorsal setae present index 5.0-6.0; pecten row slightly twisted, only 3 pairs of hair tufts index 6.0-7.0; 5 pairs of hair tufts; subdorsal setae present index 6.0-7.0; 4 pairs of hair tufts, 2nd & 3rd usually double or triple, distal usually single index 3.0-4.0; distinctly upturned; 8 pairs of hair tufts; long, curved preapical spine index 3.8-4.0; bulbous; 4 pairs of hair tufts; 2 basal tufts multi-branched index 4.0-4.5; 4 pairs of hair tufts - 3 pairs of single hairs (one offset), apical hair has 2 or 3 branches index 6.0-7.0; 4 pairs of hair tufts - 2 basal tufts have 3 branches index 6.0-7.0; 4 or 5 pairs of hair tufts
    GILLS narrow; longer than anal segment longer than anal segment blunt; 0.75-1.5 times the length of the anal segment blunt; longer than anal segment blunt point; different lengths; longer than anal segment blunt; longer than anal segment blunt; longer than anal segment blunt; longer than anal segment blunt; as long as or longer than anal segment

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    Mansonia & Coquillettidia

    characters Ma dyari Ma titillans Cq perturbans
    ANTENNAE 2 long hairs insert near middle & reach to the end 2 long hairs insert near middle & reach to the end 2 short hairs insert near middle & do not reach the end
    COMB SCALES broad; 6-9 in 1 row long, thorn-like; longer near siphon, shorter near anal segment; 6-8 in 1 row thorn-like; 8-15 in 1 irregular row
    ANAL SEGMENT lateral hair a small tuft; 4 or 5 precratal setae lateral hair a small tuft; 4 precratal setae lateral hair a long tuft; 1 or 2 tiny precratal setae
    SUBSIPHONAL HAIR 3 or 4 long, weakly barbed branches 2-3 long, strongly barbed branches 3 long strongly barbed branches
    GILLS pointed; shorter than anal segment pointed; shorter than anal segment pointed; shorter than anal segment

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    Psorophora

    characters Ps ciliata Ps columbiae Ps ferox Ps johnstonii
    ANTENNAE shorter than head shorter than head much longer than head; tuft near middle about as long as head; tuft near middle
    HEAD quadrate; top concave upper & lower hairs multiple upper & lower hairs double, long upper hair double, lower hair double or triple
    COMB SCALES thorn-like with strong spines; 12-16 in 1 curved row thorn-like with strong spines; 6 in 1 curved row thorn-like with strong spines; 6-8 in 1 curved row thorn-like with strong spines; 5-7 in 1 curved row
    SIPHON index 3.0-4.0; pecten long & hairlike; a single hair beyond pecten index 3.0; slightly bulbous; 3-6 widely spaced pecten teeth index 4.0; strongly bulbous; 3-5 widely spaced pecten teeth; tuft tiny index 2.5-3.0; strongly bulbous; 3-8 widely spaced pecten teeth; tuft tiny
    ANAL SEGMENT lateral hair has 3 or 4 branches lateral hair double or triple lateral hair multiple lateral hair small, multiple; 4-6 fan-like precratal setae
    GILLS pointed; longer than anal segment pointed; longer than anal segment pointed; longer than anal segment pointed; longer than anal segment

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    Uranotaenia

    characters Ur lowii Ur sapphirina
    HEAD hair #4 shorter than head spines, multiple hair #4 as long as head spines, double
    THORAX hair tuft #3 has 4-8 branches, >1/2 as long as hair #1 hair tuft #3 has 8-10 branches, <1/2 as long as hair #1
    SIPHON hair tuft inserts beyond pecten hair tuft inserts before last pecten tooth

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    Wyeomyia

    characters Wy mitchellii Wy vanduzeei
    ANTENNAE hair tuft small; 3 branches single hair, no tuft
    HEAD upper hair 3 or 4 branches upper hair single
    SIPHON index 4.0-5.0; many single hairs over entire siphon index 6.0; small single or double hairs, mostly dorsal
    ANAL SEGMENT lateral hair single; dorsal brush has 2 unbranched hairs lateral hair double; dorsal brush has 2 double-branched hairs
    GILLS blunt; 1-2 times length of saddle rounded; 4 times length of saddle

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